Abstract
The cerebral ganglia neurons containing molluscan insulin-related peptides were studied in two species of terrestrial snails—Helix lucorum L. and Eobania vermiculata L. by means of immunostaining and sulfide-silver intensification. The neurons stained immunopositively with the antibody raised against the C-peptide of the molluscan insulin-related peptide were labelled by a sulfide-silver staining as well. It suggests the presence of an endogenous heavy metal in molluscan insulin-containing cells. Analytical procedures suggest the presence of zinc in the cells which is known to be involved in insulin release in mammals. Surprisingly, only immunopositive but not sulfide-silver stained cells were revealed in the brain of juvenile animals suggesting the presence of an age-related difference in the metabolism of molluscan insulins. © 1997 John Wiley & Sons, Ltd.
Published Version
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