Abstract
BackgroundReactive gliosis had been implicated in injury and recovery patterns associated with hydrocephalus. Our aim is to determine the efficacy of minocycline, an antibiotic known for its anti-inflammatory properties, to reduce reactive gliosis and inhibit the development of hydrocephalus.ResultsThe ventricular dilatation were evaluated by MRI at 1-week post drugs treated, while GFAP and Iba-1were detected by RT-PCR, Immunohistochemistry and Western blot. The expression of GFAP and Iba-1 was significantly higher in hydrocephalic group compared with saline control group (p < 0.05). Minocycline treatment of hydrocephalic animals reduced the expression of GFAP and Iba-1 significantly (p < 0.05). Likewise, the severity of ventricular dilatation is lower in minocycline treated hydrocephalic animals compared with the no minocycline group (p < 0.05).ConclusionMinocycline treatment is effective in reducing the gliosis and delaying the development of hydrocephalus with prospective to be the auxiliary therapeutic method of hydrocephalus.
Highlights
Reactive gliosis had been implicated in injury and recovery patterns associated with hydrocephalus
Hydrocephalus is a common medical condition characterized by abnormalities in the secretion, circulation and resorption of cerebrospinal fluid (CSF), resulting in ventricular dilatation
Previous studies have noted that reactive astrocytosis and microgliosis are commonly found in hydrocephalus rats with neonatal congenital [1,3,4,8] and acquired obstructive [2,6,8] hydrocephalus, with a perinatal onset followed by up to 5 weeks of postnatal ventriculomegaly
Summary
Reactive gliosis had been implicated in injury and recovery patterns associated with hydrocephalus. Gliosis and neuroinflammation are prevalently associated with hydrocephalus, and reactive astrocytes and microglia have been found throughout the hydrocephalic brain, and followed by brain tissue fibrosis and function deterioration [1,2,3,4,5,6,7]. We established hydrocephalus rats model by kaolin inducing and treated them with minocycline, evaluated the ventricle enlargement by MRI. The gene expression of GFAP (glial fibrillary acidic protein, for astrocytes) and Iba-1 (ionized calcium binding adaptor molecule, for microglia) were detected to explore the effectiveness of minocycline in reducing gliosis and the possibility of drug intervention as a new treatment strategies
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