Mining secondary metabolism of achromobacter and analysis of key genes of petroleum degradation

Abstract

In this study, in order to further deepen the understanding of Achromobacter genomes in degrading petroleum, explore its secondary metabolic pathway, and make better use of this strain, the genomes of 20 Achromobacter strains were obtained from NCBI database, and analyzed by related gene information analysis software and secondary metabolite mining software. It was found that the genomes of 20 Achromobacter strains ranged in size from 5.67-7.21Mb to 7.21 MB. The GC content ranges from 63.7% to 68.2%, which contains 9 classes and 73 secondary metabolic gene clusters. The main secondary metabolic gene clusters are tetrahydropyrimidine, phosphonate, aryl polyenes, etc. The main oil degradation functional genes are alkB, etc., so the main degradation objects are short-chain and medium-length alkanes. The analysis results of this study are helpful to understand the characteristics of achromobacter genome, so as to further explore its utilization potential in bioremediation.