Abstract
An in-house protein crystallization robotic system has been designed and built to improve the rate for obtaining protein single crystals suitable for X-ray structure determination. The system uses the hanging drop, vapor diffusion technique. Positive features of previous crystallization robot designs in other laboratories were combined with several new features and requirements in the present system. The system is designed to minimize the degree of carry over from one crystallization trial to the next, to operate at 4°C, and to operate sequentially on as many as 51 Linbro 24-well culture dishes without operator intervention. The system has a number of design elements which could be adapted to other robotic crystal growth systems.
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