Abstract
Methotrexate (MTX), a structural analog of folic acid, known as one of the most potent and widely used medications for the treatment of different types of cancer and autoimmune diseases. However, the long-term treatment with MTX can cause various side effects. Calcium folinate (CF) is usually used with MTX to reduce the toxicity of MTX and enhance the efficacy of treatment. Therefore, the simultaneous determination of MTX and CF is very important. In this study a nanocomposite consisting of Fe-based metal–organic framework (MIL-101 (Fe)-NH2) and multi-walled carbon nanotubes MWCNTs) was synthesized by solvothermal method. The morphology and structure of the MIL-101 (Fe)-NH2/MWCNTs nanocomposite were investigated by various techniques. Then, a glassy carbon electrode modified with synthesized nanocomposite (MIL-101(Fe)-NH2/MWCNTs/GCE) was employed as a working electrode for voltammetric detection of MTX. Compared with un-modified GCE, the MIL-101 (Fe)-NH2/MWCNTs/GCE showed good electro-catalytic performance for oxidation of MTX. Under optimal conditions, the oxidation peak currents of MTX exhibit a linear relationship with its concentration in the range of 0.1–300.0 μM, and the limit of detection (LOD) is 0.04 μM. Also, the MIL-101 (Fe)-NH2/MWCNTs/GCE showed good activity towards MTX determination in the presence of CF. The separation of the oxidation peak potentials (200 mV), indicating its suitability for the simultaneous detection of these two drugs using differential pulse voltammetry (DPV). Finally, applicability of the developed sensor was verified by MTX and CF analysis in the pharmaceutical specimens with excellent results (recovery 96.9–103.4 %, and relative standard deviation (RSD) ≤ 3.6 %).
Published Version
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