Abstract
Studies were conducted on the ability of microfilariae (mff) of Brugia pahangi to penetrate midguts of Aedes trivittatus and Aedes aegypti (black-eyed Liverpool strain) in vitro. At 12–14 days postexposure (PE) to microfilaremic jirds, A. trivittatus exhibited a significantly lower prevalence and mean intensity of infection than A. aegypti. Nearly 50% of the mff ingested by A. aegypti, but only 7.5% of the mff ingested by A. trivittatus, penetrated through midguts within 2 hr PE. Examination of 4-μm plastic sections of midguts revealed few quantifiable differences in rate or extent of peritrophic membrane formation between the two mosquito species at 0, 30, 60, 90, or 120 min and 6, 12, or 24 hr PE. At 2 hr PE, blood meals of A. trivittatus appeared more coagulated than did blood meals from A. aegypti. Feeding both mosquito species on heparinized or citrated blood in membrane feeders resulted in significant increases in the percentage of mff penetrating the midgut of A. aegypti, but a significantly reduced percentage of mff penetrating the midgut of A. trivittatus. A significant decrease in percentage of mff penetrating midguts of both mosquito species resulted when mosquitoes were exposed to blood containing EDTA.
Published Version
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