Microwave-assisted extraction of chlorophyll and polyphenol with antioxidant activity from Pandanus amaryllifolius Roxb. in Vietnam

Abstract

Pandanus amaryllifolius Roxb., also called as pandan leaf, is distributed mainly in hot and humid climate areas. Pandan leaf, grown very popular in Vietnam, contains many biological value compounds such as chlorophyll and polyphenol with oxidation prevention activity. Microwave-assisted extraction (MAE) has advantages such as reduced extraction time and high extraction efficiency in comprison to other extraction technologies. The aim of this study was to assess the effect of microwave-assisted extraction conditions on chlorophyll and polyphenol content as well as antioxidant activity of pandan leaf. Research results show that acetone solvents of 90°, pandan powder: acetone ratio of 1:30, microwave capacity of 300W, microwave-assisted time of 2 minutes are parameters for the highest extraction efficiency. With these conditions, chlorophyll a, b and total chlorophyll content are respectively 9.4278 μg/mL, 4.2460 μg/mL, 13.6738 μg/mL, polyphenol content is 2.7577 g/L and DPPH radical collection activity 1409.51 μmol/L.