Abstract
Microwave-assisted extraction of the polysaccharide from blackcurrant (Ribes nigrum L.) fruits (MBP) was optimized using response surface methodology (RSM). The yield of MBP was 10.59 % (w/w) under the optimal conditions of power (414 W), liquid to material ratio (31/1 mL/g), and the time (41 min). After purification using D4006 macroporous resin and Sepharose-6B column chromatography, a water-soluble acidic heteropolysaccharide (PMBP) was obtained with a molecular weight of 1.99 × 106 g/mol. PMBP was thermally stable and exhibited excellent rheological properties. Fourier transform infrared spectroscopy, gas chromatography and nuclear magnetic resonance analysis confirmed the presence of α-, β-pyranose and six main glycosidic linkages in PMBP. Scanning electron microscopy, atomic force microscopy, and X-ray diffraction studies demonstrated that PMBP was amorphous aggregates with irregular shape, porous, and smooth surface. Bioactivity assays revealed the notable antioxidant and hypoglycemic activities of PMBP. Moreover, PMBP was kinetically characterized as a competitive inhibitor for α-amylase and α-glucosidase.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
