Microwave synthesis of Chitosan-stabilized selenium nanoparticles: Intrinsic oxidant scavenging capabilities, hemocompatibility, anticancer, and antibacterial potency

Abstract

Chitosan-stabilized selenium nanoparticles (Ch-St-SeNPs) were produced using Chitosan (Ch) in conjunction with microwave technology. Based on the morphological analysis, it was determined that the Ch-St-SeNPs were spherical and had sizes that varied between 8.0 and 17.0 nm. In addition, the DPPH radical scavenging test was conducted to evaluate the antioxidant effectiveness. The results showed that the green nanoparticle, prepared at 100.0 mg/mL, exhibited the highest scavenging capacity (297.1 ± 10.2 mg g−1). The concentrations of Ch-St-SeNPs reach 500 mg/ml, and the in-vitro cell viability of Ch-St-SeNPs via human breast cancer MCF-7 cell lines will decrease to 41.2 ± 3.1 % within a day of exposure. The evaluation of Ch-St-SeNPs included an analysis of their hemolytic strength, which was found to be significant. According to the findings, Ch-St-SeNPs exhibited excellent results, with no noticeable effect on RBCs and a considerably lower toxicity rate (7.2 %) compared to Triton-X-100, which showed a toxicity rate of 98.1 %. The effectiveness of Ch-St-SeNPs was evaluated by testing its antibacterial properties against various types of bacteria, including Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus), and Bacillus subtilis (B. subtilis). Results showed thatCh-St-SeNPs had a more significant impact on E. coli with a ZOI of 23.2 ± 1.3 mm compared to the standard antibiotic, which only had a ZOI of 15.0 ± 0.7 mm. According to prior research, Ch-St-SeNPs have potential applications in healthcare due to their ability as anticancer, antioxidants, and impact bacteria. In addition, the compatibility of human red blood cells is also a factor to consider.