Induction of DNA Damage and Apoptosis Mediate Cytotoxic by Oxaliplatin In Human Breast Cancer MCF-7 Cell Lines in Vitro

Abstract

Cancer is an extremely disease leading cause of human death worldwide, and breast cancer is the most common type of malignancy in women. It is a heterogenous and hormone-dependent disease. Oxaliplatin is a novel platinum derivative and is a potent chemotherapeutic agent. Therefore, oxaliplatin is an apoptotic effectual compound for treating cancer with cytotoxic side effects. To explore the underlying mechanism of action of oxaliplatin, we examined the induction of apoptosis on human breast cancer MCF-7 cell lines by the drug and introduced its possible mechanism of action. The oxidative-induced DNA damage was evaluated by 8-hydroxy-2-deoxyguanosine (8-OHdG) reduction. The cellular pathways involved in the apoptosis of P53 and BCL2 were also assessed by qPCR and Western blotting assays. The results showed that oxaliplatin exposure causes increased oxidative stress levels and activation of P53, and repression of BCL2 was also involved in these mechanistic pathways.Graphical abstract on the apoptotic mechanism of oxaliplatin via P53 activation and repression of BCL2 in MCF-7 cells.