Abstract

ABSTRACT Tetratrichomonas didelphidis is a flagellated protozoan found in the intestine of opossums. Thespecimens were stained by the Giemsa method and by FLUTAX-2, an active fluorescent derivative ofTaxol which binds to the αβ -tubulin polimerized of microtubules of cells. Giemsa stain revealed themorphological features of trichomonads such as four anterior flagella, undulating membrane, axostyleand posterior flagellum. An intense fluorescence was observed in living trophozoites of T. didelphidis and Trichomonas vaginalis (used as control), incubated with FLUTAX-2. An analysis of thecomposition of the cytoskeleton of T. didelphidis will contribute to understanding the cellularmorphology of the parasites. Key words: Tetratrichomonas didelphidis , microtubule cytoskeleton, fluorescent taxoid. INTRODUCTIONFew trichomonad species have a provenpathogenic potential for mammals and birds. Ingeneral, the species inhabiting trichomonadprimary sites, i. e., the large intestine, appear tobe non pathogenic. The species with provenpathogenic potential have evolved to inhabit areasother than the large intestine of their hosts

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