Microsphere-Based Microfluidic Device for Plasma Separation and Potential Biochemistry Analysis Applications.

Hongyan Xu,Jinan Deng,Ning Hu,Jun Qiu,Lihong Gao,Zhangying Wu,Jun Yang

doi:10.3390/mi12050487

Hongyan Xu, Jinan Deng + Show 5 more

Open Access

https://doi.org/10.3390/mi12050487

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Journal: Micromachines	Publication Date: Apr 26, 2021
Citations: 3	License type: CC BY 4.0

Affiliation: Chongqing University, Army Medical University

Abstract

The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.

Highlights

IntroductionMicromachines 2021, 12, Blood biochemical tests are widely adopted for the screening of diseases in clinical diagnosis
Removing red blood cells (RBCs) from the whole blood is important for plasma separation
Previous research has shown that the size of the microspheres should be controlled between 20 μm and 7.8 μm in order to prevent the movement of RBCs and allow the plasma to pass through at the same time [20]

Summary

Introduction

Micromachines 2021, 12, Blood biochemical tests are widely adopted for the screening of diseases in clinical diagnosis. Red blood cells (RBCs) in the whole blood, with an intense red color, can interfere with test results [1,2]. Separation of the plasma from the whole blood sample is usually prerequired for an accurate determination of the components in a blood sample [1,3]. The centrifugation technique is a common method for plasma separation in a clinical test. This technique requires bulky and expensive equipment, limiting its applications in resource-limited settings [4]. The development of a cost-effective point-of-care testing (POCT) device for plasma separation is highly desired

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Results

Conclusion