Abstract
BackgroundHuman parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) may cause intrauterine infections with potentially severe consequences to the fetus. Current serodiagnosis of these infections is based on detection of antibodies most often by EIA and individually for each pathogen. We developed singleplex and multiplex microsphere-based Suspension Immuno Assays (SIAs) for the simultaneous detection of IgG antibodies against B19V, CMV and T. gondii.MethodsWe tested the performances of SIAs as compared to in-house and commercial reference assays using serum samples from well-characterized cohorts.ResultsThe IgG SIAs for CMV and T. gondii showed good concordance with the corresponding Vidas serodiagnostics. The B19V IgG SIA detected IgG in all samples collected >10 days after onset of symptoms and showed high concordance with EIAs (in-house and Biotrin). The serodiagnostics for these three pathogens performed well in multiplex format.ConclusionsWe developed singleplex and multiplex IgG SIAs for the detection of anti-B19V,-CMV and -T. gondii antibodies. The SIAs were highly sensitive and specific, and had a wide dynamic range. These components thus should be suitable for construction of a multiplex test for antibody screening during pregnancy.Electronic supplementary materialThe online version of this article (doi:10.1186/s12879-015-1194-3) contains supplementary material, which is available to authorized users.
Highlights
Human parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) may cause intrauterine infections with potentially severe consequences to the fetus
The current standards in antibody detection are enzyme-immuno assay (EIA) and chemiluminescence immune assay (CLIA) performed individually for each pathogen; the cost of which has limited its widespread use when related to the overall low incidence of these conditions [4]
The four borderline samples had been collected within 6 days of onset; three of them were weakly positive in EIA, and one was negative
Summary
Human parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) may cause intrauterine infections with potentially severe consequences to the fetus. Current serodiagnosis of these infections is based on detection of antibodies most often by EIA and individually for each pathogen. We developed singleplex and multiplex microsphere-based Suspension Immuno Assays (SIAs) for the simultaneous detection of IgG antibodies against B19V, CMV and T. gondii. Human parvovirus B19 (B19V), cytomegalovirus (CMV) and Toxoplasma gondii (T. gondii) are ubiquitous pathogens causing diverse clinical manifestations Their primary infections during pregnancy may lead to severe sequelae. We developed Luminex-based singleplex and multiplex microsphere Suspension Immuno Assays (SIAs) to detect IgG antibodies against these three pathogens. We determined the performances of the SIAs in selected cohort groups (both in singleplex and multiplex format) in comparison to corresponding high-quality inhouse or commercial EIAs
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