Abstract
Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology. Spermatogenic cells from parasites recovered from treated and control sheep were examined by microscopy. The fecal egg-count reduction test revealed 97.06% efficacy of albendazole in the treatment of F. hepatica. Changes in testicular tubule cells started 48 hours after treatment and became evident within 72 hours, at which point it became difficult to identify cell types. Primary and secondary spermatogonia became increasingly rare and intercellular vacuolization was more evident. Signs of apoptosis, with pycnotic nuclei and evidence of keriorrexia were observed at all times. Cell debris was identified 96 hours after treatment. The results indicated that parasitic spermatogenesis was severely affected by albendazole and demonstrated the importance of the use of histopathology for the diagnosis of therapeutic efficacy in field strains.
Highlights
An adequate association between property management and flock treatment schedules is necessary for the effective control of Fasciola hepatica (FAO, 1994)
The efficacy test was performed on sheep treated with albendazole at the dose recommended for F. hepatica, in which the helminths recovered at necropsy were counted and separated for histology
The fecal egg-count reduction test (FECRT) revealed that albendazole is efficient in the treatment of F. hepatica infected sheep, where a reduction in egg count and efficacy of 97.06% could be observed (Table 1)
Summary
An adequate association between property management and flock treatment schedules is necessary for the effective control of Fasciola hepatica (FAO, 1994). When assessing the efficacy of, or resistance to, these drugs, it is important to consider the strain present in each region and even on each property. Studies related to the production, development, and/or elimination of helminth eggs in feces, the detection of antigens in feces, and the morphological and functional changes in the tegument and reproductive system of the parasite are essential for the proper demonstration of treatment results. These studies involve the collection and analysis of feces, the incubation of eggs in vitro, and scanning electron microscopy, transmission electron microscopy, or histopathology analysis
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