Microsatellites based parental polymorphism survey for moisture stress in Rice (Oryza sativa L.) between the parental genotypes HUR-1309 and CR Dhan 801

Abstract

The present investigation was conducted during Rabi 2018-19. The parental polymorphism research was done at the Molecular Drought Breeding Laboratory (Department of Genetics and Plant Breeding), Institute of Agricultural Sciences, BHU, and the Crop Improvement Division, ICAR-NRRI, Cuttack. The purpose of this research was to identify the parental polymorphism markers, analyze their chromosomal distribution, and determine the repetitive motifs. The polymorphic markers will be helpful to introgress the drought tolerant yield QTLs (qDTY) from the donor CR Dhan 801 into the background of HUR-1309, a popular aromatic short duration variety through marker-assisted backcross breeding program. Polymerase chain reaction (PCR), agarose gel electrophoresis, and genomic DNA separation were carried out according to standard procedures. A total of 510 microsatellites were used for evaluation of the parental polymorphism and 90 markers showed polymorphism among the parents and the rest 420 markers are monomorphic for the studied QTLs (qDTY1.1, qDTY2.1, and qDTY3.1). The parental polymorphism in the present study ranged between 11.43% to 30.61%. Considering the 12 chromosomes of rice, the average polymorphism was observed 17.65%. Out of 90 polymorphic markers, 59 were dinucleotide repeats, 24 were trinucleotide repeats and 4 were tetra nucleotide repeats. The dinucleotides were highly present on chromosomes 1 (12), 2 (9), and 3 (9) and repeated 12, 9, and 9 times respectively. The markers which are found to be polymorphic can be used in the marker-assisted backcross breeding (MABB) program for grain yield under moisture stress.