International Journal of Environment and Climate Change
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Parental Polymorphism Survey for Evaluation and Selection of Contrasting Parents for Drought Tolerance in Rice (Oryza sativa L.) by Using SSR Markers

Publication Date Sep 14, 2022

Abstract

Aim: This study aims to find the informative polymorphic Microsatellite (SSR) markers for drought tolerance between the recipient parent (Pratishya) and donor parent (CR Dhan 801) of rice. The drought tolerant QTLs (qDTY1.1, qDTY2.1, and qDTY3.1) from the donor parent were subsequently introgressed into the popular variety Pratikshya via marker-assisted backcross breeding. Place and Duration of Study: The parental polymorphism study was conducted at Molecular Drought Breeding Laboratory, Department of Genetics and Plant Breeding and Central Laboratory, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi; and at Crop Improvement Division, ICAR-NRRI, Cuttack during Rabi 2018-19. Methodology: The parents used for the study were Pratikshya (recurrent parent) and CR Dhan 801 (donor parent). The leaf samples were collected from 20-25 days old seedlings which were used for parental polymorphism survey after DNA extraction. The genomic DNA isolation was done by using CTAB method. A set of 510 SSR markers covering all the 12 chromosomes of rice were used for parental polymorphism survey in the present study. The standard protocol of polymerase chain reaction (PCR) and agarose gel electrophoresis was used. The DNA fragments were documented using gel documentation system. The graphical representation of marker data was obtained with GGT 2.0 software. Results: Out of 510, total 108 SSR primer pairs exhibited polymorphism between recipient parent (Pratikshya) and donor parent (CR Dhan 801) and remaini...

Concepts

Donor Parent Parental Polymorphism Institute Of Agricultural Sciences Markers For Drought Tolerance Recipient Parent Parental Polymorphism Survey Drought Tolerance In Rice Gel Documentation System Marker-assisted Backcross Breeding Banaras Hindu University

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