Abstract
The aim of this study was to develop PCR-based suitable microsatellite marker panels for paternity testing and to define pedigree errors in Kilis goats. A total of 137 head of goats were used, consisting of 118 head of kids and 19 head of possible candidate sires. A total of 392 alleles were observed in 22 microsatellite markers. Allele numbers ranged between 12 (SRCRSP7) and 24 (BM1818, INRA0023, and SRCRSP15) with an average of 17.82. The mean value of the effective allele numbers was obtained as 9.44. The overall polymorphic information content value was quite high (0.88). The overall observed (Ho) and expected heterozygosity (He) values for all studied loci were 0.89 and 0.89, respectively. Paternity test panels for the Kilis goat population studied were created based on individual probability of exclusion of microsatellites in multiplex groups. Combined probability of exclusion (CPE) values for different panels ranged between 0.745 (Panel-1) and 0.9999 (Panel-22), while the combined identification probability (CPI) values were obtained in the range of 9.81 × 10$^{-3}$ (Panel-1) to 6.96 × 10$^{-21}$ (Panel-22). As a result of this study, it can be stated that panels with 0.999 CPE values can be used at the most reasonable cost and with high reliability in paternity tests to be performed in Kilis goats, which can be a reference for other populations.
Highlights
Identification and validation of the biological father of a living thing by methods such as blood group, hemoglobin typing, transferrin typing, or DNA analysis is defined as a paternity test
When the numbers of alleles (Na), Ne, mean number of alleles (MNa), and polymorphic information content (PIC) values obtained in this study are examined, it is noteworthy that the microsatellites used have very high polymorphism
Obtained MNa and PIC values were significantly higher than those of some other Turkish goat breeds and other goat breeds raised in different countries [17,18], while these values were lower than those of goat breeds raised in North Africa [19]
Summary
Identification and validation of the biological father of a living thing by methods such as blood group, hemoglobin typing, transferrin typing, or DNA analysis is defined as a paternity test. Paternity tests are used to identify and verify this type of information [1]. Because they contain a high level of information, microsatellite markers are used widely in paternity tests to control pedigree records. The high information content of microsatellite markers depends on the number of alleles given by the marker and the frequencies in the corresponding populations [2]. The accuracy of pedigree information in many animal species is one of the most important steps for breeding programs. Pedigree errors have great influence on the answer to the selection in the breeding programs [3]. Incorrect selection of male stock candidates in animal breeding programs will result in lower genetic progress than expected [1,4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
