Abstract
Fusarium head blight is one of the most widespread and dangerous wheat diseases worldwide. Resistance to Fusarium is controlled by some main genes from different Triticinae genomes; however, common wheat has few such resistance genes. Miguschova wheat (Triticum miguschovae Zhir.) with AbAbGGDD genome is characterized by the genotype resistant to Fusarium head blight. In order to effectively use it as a source of introgressions to common wheat genome, molecular genetic polymorphisms should be identified, which could later be used for identification of Miguschova wheat introgressions in the common wheat genome. Microsatellite PCR analysis using primers to SSR loci with a known chromosome localization for common wheat identified 14 highly informative loci with specific to Miguschova wheat amplicons, localized on chromosomes of 6 homoeological groups. Seven other SSR loci were identified to have a limited informative value, as DNA of Miguschova wheat did not form any specific PCR product with corresponding primers (null allele). The informative value of those loci was limited to differentiation of wheat cultivars. Manuscript received 16.04.2020
Highlights
Fusarium head blight is one of the most widespread and dangerous wheat diseases worldwide
The article offers the results of a comparative microsatellite analysis of genomes of Miguschova wheat and common wheat cultivars, and these results could be the basis for selection of cross combinations perspective for the future work with their derivatives
Search for polymorphism between Miguschova wheat and common wheat cultivars were conducted through comparison of electrophoretic spectra of amplification products obtained using primers to microsatellite loci with chromosome specificity for common wheat
Summary
Fusarium head blight is one of the most widespread and dangerous wheat diseases worldwide. The use of this species for development of introgressive lines with common wheat as a recipient genome (T. aestivum L, AABBDD genome) is promising for enrichment of common wheat with genes for resistance to Fusarium head blight. Use of microsatellite (SSR) loci for identification of introgressions in wheat is widespread and productive direction for screening introgressive derivatives of distant crosses [5,6,7,8]. Search for polymorphism of chromosome specific SSR loci in components of initial cross (common wheat x Miguschova wheat) is a key stage in preparation for screening introgressive progeny, as on the presence of such polymorphism depends possibility of the following selection of those hybrids that contain introgressions in their genomes. The article offers the results of a comparative microsatellite analysis of genomes of Miguschova wheat and common wheat cultivars, and these results could be the basis for selection of cross combinations perspective for the future work with their derivatives
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