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Abstract
This article describes development of multilocus SSR-markers sets for genotyping Pyrus, Prunus, and Malus from various genetic collections of the South of Russia. Generated multiplex sets of SSR-markers were used in the certification of cultivated varieties and in the analysis of the genetic structure of Pyrus, Prunus and Malus species collections. The results of SSR genotyping of pear, apple, plum and sweet cherry made it possible to establish genetic relationships between varieties, including groups of modern varieties of Russian and foreign breeding and, in turn, local autochthonous varieties. In general, the use of these multiplexes has confirmed their effectiveness in solving the assigned tasks.
Highlights
At the present stage of development of selection and genetic research of the gene pool of cultivated plants, including fruit crops, special attention is paid to the use of methods that allows in a short time and with a high level of accuracy to characterize the studied gene pool
In order to reduce the time spent in performing genotyping of a larger number of samples and to simplify the algorithm of work, one or two unified PCR protocols were developed for all SSR markers used in the work and, for all multiplex sets
The used DNA markers were grouped into multiplex sets according to the size of the amplified fragments in such a way that SSR markers with non-overlapping ranges of amplified sequence sizes were included in each set
Summary
At the present stage of development of selection and genetic research of the gene pool of cultivated plants, including fruit crops, special attention is paid to the use of methods that allows in a short time and with a high level of accuracy to characterize the studied gene pool. In 2019, a group of researchers reported on the results of genotyping of local plum species in Tunisia, genetic diversity was assessed by the polymorphism of microsatellite loci and the S-gene [12], another research team in a 2019 article outlined the results of a genetic study of almonds from various ecological-geographical regions [13]. It is worth noting a number of studies using SSR markers carried out on sweet cherry genotypes and cherry species, including the work of Russian scientists [14, 15, 16]. - performing DNA-certification based on the analysis of microsatellite loci and studying the genetic structure of the gene pool of fruit crops
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