Abstract
The Geisenheim collection contains a number of old traditional grapevines obtained during the last century from many countries including wild grapevine accessions. Over 60 samples originating from Azerbaijan, Bulgaria, Dagestan, Egypt, Greece, Hungary, Kazakhstan, Lebanon, Moldova, Romania, Russia, Turkey, Ukraine and Uzbekistan were probed for analysis. Additionally 25 accessions of wild grapevines some acquired in Germany were included to the tested panel. Accessions were analysed on 9 microsatellite loci (VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62 and VrZAG79) for standard grapevine identification done in 4 multiplex PCRs. We obtained 13.56 overall average alleles per locus (12.44 in cultivated and 7.56 in wild grapevines). Expected and observed heterozygosity in cultivated grapevines were 0.826 and 0.644, while among wild accessions it was 0.693 and 0.464 respectively. The most informative locus proved to be VVMD28 in Vitis vinifera L. ssp. sativa and VVMD7 within V. vinifera L. ssp. sylvestris GMELIN. Microsatellite profiling will enable proper identification of cultivars by obtaining groups of synonyms and homonyms through comparative analysis as well assessment future estimation of relatedness between cultivated and wild accessions.
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