Abstract

The incidence of cutaneous squamous cell carcinoma (cSCC) has been increasing in recent years. Meanwhile, microRNAs have been found to play vital roles in various cancers, including cSCC. This study aimed to investigate the expression of microRNA-573 (miR-573) in cSCC, its relationship with long non-coding RNA PICSAR and analyze its biological role. The relationship between PICSAR and miR-573 was confirmed by dual-luciferase reporter assay and Pearson’s correlation coefficient analysis. The levels of PICSAR and miR-573 were measured using quantitative Real-Time polymerase chain reaction. Cell Counting Kit-8 assay was used to evaluate the cSCC cell proliferation ability. The migration and invasion abilities of cSCC cells were evaluated by Transwell assay. PICSAR expression was increased and miR-573 was decreased in tumor tissues and cSCC cell lines. PICSAR and miR-573 can bind directly, and miR-573 expression was downregulated by PICSAR in cSCC. Overexpression of miR-573 significantly inhibited the proliferation, migration, and invasion abilities of A431 and SCC13 cells. In addition, miR-573 overexpression reversed the promotion effects of PICSAR overexpression on cSCC cell proliferation, migration, and invasion abilities. In conclusion, our findings indicated that miR-573 expression was decreased in tumor tissues and cSCC cells and was downregulated by PICSAR in cSCC. Additionally, miR-573 overexpression inhibited cSCC cell proliferation, migration and invasion, and reversed the promotion effects of PICSAR overexpression on cSCC cell biological functions. Thus, miR-573 might function as a tumor suppressor and might be involved in the regulatory effects of PICSAR on tumorigenesis in cSCC.

Highlights

  • Cutaneous squamous cell carcinoma is the second most common cancer in humans with an increasing incidence [1]

  • Chi-square test was used to analyze the association of PICSAR and miR-573 expression with the clinical characteristics of Cutaneous squamous cell carcinoma (cSCC) patients

  • This study revealed that miR-573 overexpression reversed the promotion effects of PICSAR on cSCC cell proliferation, migration and invasion

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Summary

Introduction

Cutaneous squamous cell carcinoma (cSCC) is the second most common cancer in humans with an increasing incidence [1]. Because of the sunlight or trauma, exposure to chemical agents, chronic wounds or papillomavirus infection, pre neoplastic lesions arise in the skin, which cause abnormal proliferation of keratinocytes and eventually leading to cSCC [2]. The clinical behavior of cSCC is generally benign, it may undergo local invasion and metastasis [3]. Squamous cell carcinoma itself is a more aggressive cancer, which is prone to lymph node and distal metastasis, and once metastatic, it is difficult to treat and has a poor prognosis. It is urgent to search for new diagnostic biomarkers and thereby improve the cSCC treatment outcome

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