MicroRNA-203 suppresses cell proliferation and migration by targeting BIRC5 and LASP1 in human triple-negative breast cancer cells

Chen Wang,Yurong Shi,Xiangqian Zheng,Chunyan Shen

doi:10.1186/1756-9966-31-58

Abstract

BackgroundThis study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC).MethodsReal-time PCR was performed to detect the expression of miR-203 in TNBC cell lines. miR-203 precursor and control microRNA (miRNA) were transfected into triple-negative breast cancer (TNBC) cell lines and the effects of miR-203 up-regulation on the proliferation and migration of cells were investigated. Meanwhile, the mRNA and protein levels of baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) were measured. Luciferase assays were also performed to validate BIRC5 and LASP1 as miR-203 targets.ResultsBoth miR-203 and BIRC5 siRNA signicantly inhibited cell proliferation in TNBC cells. Both miR-203 and LASP1 siRNA signicantly inhibited cell migration in TNBC cells, also. Moreover, up-regulated of BIRC5 and LASP1 was able to abrogate the effects induced by transfection with the miR-203 precursor.ConclusionsThese data suggest that miR-203 may function as a tumor suppressor in TNBC cells. Thus, miR-203 could be a potential therapeutic target for this disease.

Highlights

This study was performed to investigate the effect of microRNA-203 on cell proliferation and migration in triple-negative breast cancer (TNBC)
Results miR-203 expression was decreased in TNBC cell lines while baculoviral IAP repeat-containing protein 5 (BIRC5) and Lim and SH3 domain protein 1 (LASP1) expression was increased We detected the abundance of miR-203 in triplenegative human breast cancer cell lines: MDA-MB-468 and MDA-MB-231 and a normal breast cell line: MCF10A, by real-time PCR
It was intriguing that in sharp contrast to the down-regulation of miR-203 in TNBC cells, BIRC5 and LASP1 expression is increased in TNBC cell lines compared to that in MCF-10A (Figure 1B&C)

Summary

Introduction

This study was performed to investigate the effect of microRNA-203 (miR-203) on cell proliferation and migration in triple-negative breast cancer (TNBC). Triple-negative breast cancer (TNBC) is defined histologically as invasive carcinoma of the breast that lacks staining for estrogen receptor (ER), progesterone receptor (PgR), and the human epidermal growth factor receptor-2 (HER2). MiRNAs regulate gene expression post-transcriptionally, by pairing with complementary nucleotide sequences in the 3’-UTRs of specific target mRNAs [2,3]. This recently identified type of gene regulators is involved in modulating multiple cellular pathways, including cell proliferation, differentiation, and migration. The deletion or epigenetic silencing of a miRNA that normally represses the expression of one or more oncogenes might lead to carcinogenesis, tumor growth and invasion, as has been demonstrated for miR200, miR-122 and miR-203 [8,9,10]

Methods

Results

Conclusion