Abstract
BackgroundMicroRNAs play key roles in host-pathogen-interactions and disease pathogenesis. Our aim was to characterize the differentially expressed miRNAs in the blood cells of diseased (Brucellosis-positive, Johne’s disease-positive) and healthy- water buffaloes. The pooled small-RNA samples of each group were sequenced on Ion Torrent Personal Genome Machine (PGM) sequencer and the data were analyzed for differential expression.ResultsHere we identified 274 known miRNAs with bovine homologs and 36 novel mature-star miRNAs from the sequnces of small RNA libraries. Overall 195 miRNAs were common to all the three groups. Certain miRNAs such as bta-miR-21-5p, −26a, −29a/b, −30d − 103, − 140, − 150, − 191, − 374, − 1434-5p,-1260b, − 2484 and let-7 members were abundantly expressed in diseased groups. Bta-miR-1434-5p, − 188, −200c were up-regulated (> 1.5 folds) while bta-miR-27a-5p, −34b and -2285x were down-regulated (> 100 folds) in Brucellosis group. In Johne’s Disease group, only 3 miRNAs (bta-miR-1434-5p, − 2340 and − 2484) were up-regulated (> 1.5 folds). The functional classification of miRNA target genes into gene ontology (GO) terms indicated their involvement in innate immunity and cellular process of disease pathogenesis. Expression profile of four differentially expressed miRNAs (bta-miR-9-5p, − 677, − 331-3p and − 2440) and eight predicted target-genes were validated through reverse transcriptase qPCR.ConclusionThis study provides a valuable frame of reference for elucidation of regulatory roles of miRNAs associated with disease pathogenesis in water buffaloes as well as identification of miRNA biomarkers for disease diagnosis and treatment.
Highlights
MicroRNAs play key roles in host-pathogen-interactions and disease pathogenesis
The goals of this study were to identify peripheral blood mononuclear cells (PBMC) miRNAs profiles in Indian water buffalo associated with economically devastating diseases, namely, brucellosis and paratuberculosis (Johne’s disease or JD)
Animals Diseased vis-a-vis healthy water buffaloes (Bubalus bubalis) included in the study were from the dairy farm of our institution i.e. Guru Anagad Dev Veterinary and animal Sciences University (GADVASU), Ludhiana as well as other dairy farms situated in Punjab, based on the history of occurrences of Johne’s disease and Brucellosis
Summary
MicroRNAs play key roles in host-pathogen-interactions and disease pathogenesis. Our aim was to characterize the differentially expressed miRNAs in the blood cells of diseased (Brucellosis-positive, Johne’s diseasepositive) and healthy- water buffaloes. The goals of this study were to identify PBMCs miRNAs profiles in Indian water buffalo associated with economically devastating diseases, namely, brucellosis and paratuberculosis (Johne’s disease or JD). Brucellosis an important zoonotic disease of livestock is caused by facultative, gram-negative bacteria of Brucella genus. This (2020) 2:8 its associated lymph nodes and spreads to bloodstream, milk and other peripheral tissues [8]. MAP being an obligate intracellular pathogen resides in host macrophages and enhance its survival by inhibiting intracellular phagosomal activation and maturation [9, 10]. Brucellosis and JD, causes substantial economic losses to livestock worldwide and scientific research has been focused on their diagnosis, prevention, and control
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