Abstract
MicroRNAs (miRNAs) are small, abundant, non-coding RNAs that modulate gene expression by interfering with translation or stability of mRNA transcripts in a sequence-specific manner. A total of 734 precursor and 996 mature miRNAs have so far been identified in the chicken genome. A number of these miRNAs are expressed in a cell type-specific manner, and understanding their function requires detailed examination of their expression in different cell types. We carried out deep sequencing of small RNA populations isolated from stimulated or transformed avian haemopoietic cell lines to determine the changes in the expression profiles of these important regulatory molecules during these biological events. There were significant changes in the expression of a number of miRNAs, including miR-155, in chicken B cells stimulated with CD40 ligand. Similarly, avian leukosis virus (ALV)-transformed DT40 cells also showed changes in miRNA expression in relation to the naïve cells. Embryonic stem cell line BP25 demonstrated a distinct cluster of upregulated miRNAs, many of which were shown previously to be involved in embryonic stem cell development. Finally, chicken macrophage cell line HD11 showed changes in miRNA profiles, some of which are thought to be related to the transformation by v-myc transduced by the virus. This work represents the first publication of a catalog of microRNA expression in a range of important avian cells and provides insights into the potential roles of miRNAs in the hematopoietic lineages of cells in a model non-mammalian species.
Highlights
MicroRNAs are a large class of endogenous noncoding RNAs 21–23 nucleotides in length
MiRNA EXPRESSION IN B-LYMPHOCYTES AFTER CD40L STIMULATION Naïve B cells are activated through a combination of signals from the antigen and through the binding of the CD40 ligand to CD40 which drives proliferation
Deep sequencing using Illumina platform can be valuable for obtaining miRNAome data, and we have used this for determining the miRNA expression levels from cell lines of chicken, a model avian species
Summary
MicroRNAs (miRNAs) are a large class of endogenous noncoding RNAs 21–23 nucleotides in length. Since they were first described nearly 20 years ago, there has been a steady increase in their discovery and the latest Release 20 of miRBase has 24,521 entries of miRNAs from various species, including 734 mature miRNAs from Gallus gallus Many of these miRNAs are expressed differentially during development in a cell-specific manner. Studies examining the global expression of miRNAs in different haemopoietic cell lineages in chickens have not yet been carried out
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