Abstract
MicroRNAs (miRNAs) play essential roles in regulating gene expression involved in various biological functions. The knowledge of miRNA expression profiles across different tissues in cattle is still limited. Using the miRNAs data generated from 158 samples in three studies, we characterized the miRNA expression profiles of bovine sera, exosomes and 11 different tissues. Totally 639 miRNAs were identified and 159 miRNAs were expressed in all samples. After relative log expression normalization, four miRNA expression clusters were generated: 1) sera and exosomes; 2) liver; 3) mammary gland; 4) rumen and gut tissues. The top 10 most abundant miRNAs accounted for >55% of total miRNA expression in each tissue. In addition, this study described a detailed pipeline for identification of both tissue and circulating miRNAs, and the shareable datasets can be re-used by researchers to investigate miRNA-related biological questions in cattle. In addition, a web-based repository was developed, which enables researchers to access the distribution range and raw counts number of the miRNA expression data (https://www.cattleomics.com/micrornaome).
Highlights
Background & SummaryMicroRNAs are endogenous short non-coding RNAs (typically contain 18-25 nucleotides)that play a vital role in regulating gene expression at the post-transcriptional level by inducing the degradation or blocking translation of target messenger RNA1
Bovine miRNA studies have proposed their significant roles in diverse molecular functions and biological processes including mammary gland development[4,5,6], rumen development[7,8], ovary development[9,10], adipogenesis[11,12], infection and immunity[13,14,15], production traits improvement, early diagnosis of pregnancy[18,19,20] and so on
The Gene Expression Omnibus (GEO) records of studies 1 and 2 were the first time to be released under this study (Data Citation 1 and Data Citation 2), and GEO record of study 3 has already been released with the previous publication (Data Citation 3)
Summary
MicroRNAs (miRNAs) are endogenous short non-coding RNAs (typically contain 18-25 nucleotides (nt)). The 158 samples were derived from five different dairy cow tissues (study 1)[17], six different beef cattle tissues (study 2)[22], as well as dairy cow sera and exosomes (study 3)[23], which were all used the same library construction method (TruSeq Small RNA Sample Preparation kit) and high-throughput sequencing platform (Illumina HiSeq 2000 system) to identify the miRNA expression profiles (Fig. 1). Expression of bta-miR-2284h-5p, bta-miR-2284e, bta-miR-2285y, bta-miR-208b, bta-miR-2457, and bta-miR-7857 were only detected in the circulating fluids (Fig. 2b) and ten specific miRNAs were only identified in sera: bta-miR-935, bta-miR-2285ac, bta-miR-2454-3p, bta-miR-2388-3p, btamiR-2285l, bta-miR-599, bta-miR-2284m, bta-miR-2453, bta-miR-187, and bta-miR-371. This study describes the comprehensive datasets of multi-tissue miRNAome, which provides a valuable resource for bovine functional genomics research and can be re-used by the research community to address additional biological questions on miRNA expression profiles in cattle. The information provides applicable clues for the understanding of the roles of miRNAs in tissue development and tissue-specific functions contributing animal production and health
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
