Analysis on micro RNA expression profile relating to rats apoptosis due to acute pancreatitis

Abstract

Objective To analyze micro RNA (miRNA) expression profile relating to rats apoptosis due to acute pancreatitis.Methods Thirty-six SD rats are randomly divided into:blank control group,acute edematous pancreatitis (AEP) and acute necrosis pancreatitis (ANP).AEP group model is established by L-arginine(150mg/kg) intraperitoneal injection and ANP group model is established by L-arginine (2400 mg/kg) intraperitoneal injection;inspect every group's serum amylase and lipase content 12 h after molding;inspect the apoptosis of acinar cell in pancreatic tissue by TUNEL method and give the pancreatic tissue the pathological score;choose 2 from every group to inspect miRNA expression by miRNA chip technology,and screen out the miRNA with differential expression and make hierarchical clustering analysis.Results Compared with ANP group,AEP group's serum amylase,lipase and pathological score decline dramatically (P＜0.05 ),and apoptosis index of pancreatic acinar cells increases significantly (P＜0.05 );there are dramatic differences between the experimental group indexes and blank control group indexes (P ＜0.05 ).miRNA chip analysis:In comparison with blank control group,the expression differences of ANP group and AEP group are both more than 2 times;in comparison with ANP group,the expression difference of AEP group is more than 2 times,and the miRNA with dramatic difference (P ＜0.05 ) by statistical analysis is regarded as apoptosis associated miRNA ( AA miRNA).There are 8 AA miRNAs with expression difference,and compared with ANP group,5 increase more than 2 times;3 reduce more than 2 times.Conclusion In the process of acute pancreatitis,pathophysiologic changes are associated with miRNA expression profile,and some miRNA may participate in the regulation of pancreatic acinar cell apoptosis process. Key words: Acute pancreatitis; Apoptosis; microRNA; Rat