MicroRNA-608 Regulates Epithelial-To-Mesenchymal Transition to Inhibit Malignant Behaviors of Glioma Cells Through Nuclear Factor I-C Signaling Pathway

Abstract

MiR-608 is expressed in bladder cancer, colon cancer, prostate cancer risk, and exerts inhibitory role in gastric cancer and invasion of three negative malignant breast cancer by inhibiting the NIFC, but its role in the biology of glioma is unclear, so the purpose of this study was to explore MiR-608’s role in glioma, and further explore whether NFIC signal pathways are involved in the role of MiR-608 in glioma. We obtain glioma tissues and normal tissues to detect the expression of miR-608 by PCR. miR-608 mimics transfection or joint with NFIC expression plasmid were transfected into A172, and LN229 glioma cells followed by analysis of cell proliferation, clone formation, invasion and migration, and cell apoptosis. Compared to normal tissue, miR-608 expression in glioma tissues was decreased. After transfection of miR-608 mimics, miR-608 level, cell proliferation activity, invasion and migration activity increased significantly, and apoptosis reduced, in addition, the dual luciferase report gene and protein imprinting analysis showed NFIC to be a target of miR-608. NFIC transfection reversed miR-608’s role in glioma cells. In conclusion, microRNA-608 inhibits malignant invasion and migration of glioma cells via NFIC signaling.