Abstract
BackgroundSepsis leads to severe inflammatory and cardiac dysfunction. This study aimed to explore the clinical value of miR-495 in sepsis, as well as its role in sepsis-induced inflammation and cardiac dysfunction.Methods105 sepsis patients were recruited; receiver operating characteristic (ROC) curve was plotted to assess the diagnostic value of miR-495 in sepsis. A model of sepsis in rats was created via performing cecal ligation and puncture (CLP). After modeling, the cardiac function, including left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and maximum rate of rise/fall of left ventricle pressure (± dp/dtmax), and serum cardiac troponin I (CTn-I), creative kinase isoenzyme MB (CK-MB) were detected. The blood cytokines levels including TNF-α, IL-6, IL-1β were also measured. Quantitative real-time PCR (qRT-PCR) was used for the measurement of the expression level of miR-495.ResultsMiR-495 was significantly downregulated in sepsis patients, especially patients who suffered from septic shock (SS). MiR-495 expression was negatively associated with Scr, WBC, CRP, PCT, APACHE II score and SOFA score. MiR-495 could distinguish patients with SS from non-SS patients. MiR-495 and SOFA score were better indictors for the occurrence of cardiac dysfunction in sepsis patients. In CLP-induced sepsis model. CLP rats experienced deterioration of LVSP, LVEDP, ± dp/dtmax, and had a rise in serum CTn-I, CK-MB, TNF-α, IL-6 and IL-1β, which were improved by miR-495 agomir injection.ConclusionsMiR-495 might be a potential diagnostic biomarker for sepsis patients, and overexpression of miR-495 alleviated sepsis-induced inflammation and cardiac dysfunction.
Highlights
Sepsis leads to severe inflammatory and cardiac dysfunction
Wang et al [18] reported that miR495 is involved in the regulation of cardiac fibrosis, which plays a crucial role in sepsis-induced cardiac dysfunction
The serum levels of serum creatinine (Scr), white blood cell (WBC), C-reactive protein (CRP) and PCT were higher in sepsis patients than that in healthy controls, whereas the serum albumin level was lower in patients group, and all differences reached
Summary
Sepsis leads to severe inflammatory and cardiac dysfunction. This study aimed to explore the clinical value of miR-495 in sepsis, as well as its role in sepsis-induced inflammation and cardiac dysfunction. Sepsis can lead to a severe systemic inflammatory response, even shock [2]. It has been reported that cardiac dysfunction is a major complication of. Guo et al Eur J Med Res (2019) 24:37. Recent studies have already suggested the involvement of miR-495 in the inflammatory response of several human diseases, such as ankylosing spondylitis (AS), inflammatory bowel disease [16, 17]. Wang et al [18] reported that miR495 is involved in the regulation of cardiac fibrosis, which plays a crucial role in sepsis-induced cardiac dysfunction. The role of miR-495 in sepsis has not been reported
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