Abstract
Sepsis often leads to cardiac dysfunction and inflammation. This study investigated the clinical value of microRNA-328 (miR-328) in sepsis and its role in cardiac dysfunction and inflammation caused by sepsis. The expression level of miR-328 in the serum of the subjects was detected by qRT-PCR. Receiver operating characteristic (ROC) curve measured the diagnostic value of miR-328 in sepsis. Rat sepsis model was established to detect left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), and maximal rate of increase/decrease of left ventricular pressure (±dp/dtmax). Myocardial injury markers serum cardiac troponin I (cTnI), myocardial kinase isoenzyme (CK-MB), and inflammatory factors were detected by enzyme-linked immunosorbent assay (ELISA). miR-328 expression was assessed in serum of sepsis patients and in rat models of sepsis. The AUC of ROC curve was 0.926, sensitivity 87.60%, and specificity 86.36%. Compared with the sham group, LVSP and +dp/dtmax were decreased in the rat model of sepsis. LVEDP, -dp/dtmax, cTnI, CK-MB, tumor necrosis factor-α, interleukin (IL)-6, and IL-1β were upregulated in the rat model of sepsis. The low expression of miR-328 reversed these indicators. miR-328 is a diagnostic marker for patients with sepsis, and decreasing the expression level of miR-328 can ameliorate cardiac dysfunction and cardiac inflammation in sepsis.
Highlights
Sepsis has been identified as a systemic inflammatory response to infection or injury [1]
There was no significant difference in age (Po0.150), gender (Po0.768), and BMI (Po0.258) between the healthy control group and the sepsis patients group, but significant differences in Scr, WBC, C-reactive protein (CRP), and PTC were observed (Po0.001)
The experimental results showed that the expression level of miR-328 was significantly positively correlated with the condition of sepsis (Table 2)
Summary
Sepsis has been identified as a systemic inflammatory response to infection or injury [1]. About 50% of sepsis patients require intensive care unit treatment [2,3], leading to high morbidity and mortality [4,5]. Cardiac dysfunction is one of the common complications of sepsis-induced death [8,9]. Myocardial injury and myocardial depression are the most common cardiac dysfunctions caused by sepsis [10]. As a systemic inflammatory response, inflammation-related serum C-reactive protein (CRP), procalcitonin (PTC), and interleukin (IL-6) have been used in the diagnosis of sepsis [12,13], but their specificity and sensitivity are limited by different conditions. New biomarkers are urgently needed for the early diagnosis and accurate assessment of sepsis patients
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