Abstract
With the development of information technology, the concept of smart healthcare has gradually come to the fore. Smart healthcare uses a new generation of information technologies, such as the Internet of Things (loT), big data, cloud computing, and artificial intelligence, to transform the traditional medical system in an all-around way, making healthcare more efficient, more convenient, and more personalized. miRNAs can regulate the proliferation, differentiation, and apoptosis of human cells. Relevant studies have also shown that miRNAs may play a key role in the occurrence and development of myocardial ischemia-reperfusion injury (MIRI). This study aims to explore the effects of miR-489 in MIRI. In this study, miR-489 expression in a myocardial ischemia-reperfusion animal model and H9C2 cells induced by H/R was detected by qRT-PCR. The release of lactate dehydrogenase (LDH) and the activity of creatine kinase (CK) was detected after miR-489 knockdown in H9C2 cells induced by H/R. The apoptosis of H9C2 cells and animal models were determined by ELISA. The relationship between miR-489 and SPIN1 was verified by a double fluorescence reporter enzyme assay. The expression of the PI3K/AKT pathway-related proteins was detected by Western blot. Experimental results showed that miR-489 was highly expressed in cardiac muscle cells of the animal model and in H9C2 cells induced by H/R of the myocardial infarction group, which was positively associated with the apoptosis of cardiac muscle cells with ischemia-reperfusion. miR-489 knockdown can reduce the apoptosis of cardiac muscle cells caused by ischemia-reperfusion. In downstream targeting studies, it was found that miR-489 promotes the apoptosis of cardiac muscle cells after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. In conclusion, high expression of miR-489 is associated with increased apoptosis of cardiac muscle cells after ischemia-reperfusion, which can promote the apoptosis after ischemia-reperfusion by targeting the inhibition of the SPIN1-mediated PI3K/AKT pathway. Therefore, miR-489 can be one of the potential therapeutic targets for reducing the apoptosis of cardiac muscle cells after ischemia-reperfusion.
Highlights
Acute coronary syndrome (ACS) is a group of clinical syndromes caused by acute myocardial ischemia due to coronary thrombosis, with high mortality
To further clarify the effects of miR-489 on the apoptosis of cardiac muscle cells caused by ischemia-reperfusion, H9C2 cells induced by H/R after cell transfection were divided into control group, miR-489 negative control (NC) group, and miR-489 inhibitor group, and miR-489 expression and apoptosis in the three groups were measured, respectively
In order to investigate the influence of miR-489 on the apoptosis of cardiac muscle cells in myocardial ischemia-reperfusion injury (MIRI) and its specific mechanism, we established a myocardial ischemia-reperfusion model in rats to explore the relationship between miR-489 expression and the apoptosis of cardiac muscle cells after myocardial ischemia-reperfusion. e results showed that the levels of LDH and CK in the serum significantly increased in the H/R group, and miR-489 expression significantly increased in cardiac muscle cells in the infarction border zone, compared with those of the control group
Summary
Acute coronary syndrome (ACS) is a group of clinical syndromes caused by acute myocardial ischemia due to coronary thrombosis, with high mortality. Restoration of the effective blood flow in cardiac muscles can significantly reduce the cardiac cell injury caused by ischemia and fully improve the prognosis of patients, which is the first-line treatment protocol for ACS [1]. Is tissue injury caused by the recovery of blood flow after ischemia in cardiac muscle cells is called myocardial ischemia-reperfusion injury (MIRI), which may cause irreversible injury to the structure and functions of cardiac muscles, as an important factor affecting the prognosis of patients with ACS. Erefore, it is crucial to clarify the factors affecting the apoptosis of cardiac muscle cells after myocardial ischemia-reperfusion. It has been gradually recognized that microRNA may play a key role in the occurrence and development of MIRI, associated with myocardial autophagy, apoptosis, necrosis, and oxidative stress, inflammation, and mitochondrial dysfunction [5,6,7,8]. Biological functions, and potential mechanism in ischemia-reperfusion are not completely clear. is study aims to explore the effects of miR-489 in MIRI and its specific mechanism to provide a reference for the treatment of MIRI
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