Abstract
Renal cell carcinoma (RCC) is the most common type of malignancy in the kidney parenchyma. MicroRNAs (miRNAs) are small non‑coding RNAs that serve a role in various biological processes associated with human cancer. The present study aimed to explore the potential role of miRNA (miR)‑373 in the tumorigenesis of RCC. The effects of miR‑373 on the proliferation and apoptosis of RCC cells were determined using MTT, colony formation and flow cytometry assays invitro. The results demonstrated that miR‑373 was significantly upregulated in RCC tissues and cell lines. Knockdown of miR‑373 expression reduced cell proliferation and promoted cell apoptosis in 786‑O and ACHN cell lines. Furthermore, an invivo tumorigenicity assay revealed that knockdown of miR‑373 expression reduced tumor growth in nude mice. Taken together, these data indicate that miR‑373 may promote tumorigenesis in RCC, suggesting that miR‑373 may act as a potential therapeutic target against RCC.
Highlights
MicroRNAs are endogenous small non‐coding RNA molecules, ~22 nucleotides in length, which can regulate gene expression at the transcriptional or post‐transcriptional level by binding with the 3'‐untranslated regions of target mRNAs [1,2,3]
Results miR‐373 expression is significantly upregulated in Renal cell carcinoma (RCC) tissues and cell lines
To determine whether miR‐373 expression is associated with RCC development, the expression levels of miR‐373 in 52 pairs of RCC and adjacent normal tissues were detected using Reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR)
Summary
MicroRNAs (miRNAs) are endogenous small non‐coding RNA molecules, ~22 nucleotides in length, which can regulate gene expression at the transcriptional or post‐transcriptional level by binding with the 3'‐untranslated regions of target mRNAs [1,2,3]. Previous studies have indicated that miRNAs are aberrantly expressed in various types of human cancer [7,8,9]; miRNAs may act as oncogenes or tumor suppressors in cancer depending on their tissue‐ and disease‐specific expression patterns [10,11]. In cancer, downregulated miRNAs usually serve as tumor suppressors, whereas upregulated miRNAs act as oncogenes. Renal cell carcinoma (RCC) is the most common type of adult kidney tumor worldwide, and accounts for 2‐3% of all adult malignancies [12,13]. Improved understanding regarding the molecular mechanisms underlying the pathogenesis and development of RCC, and developing more effective treatment options for the treatment of RCC, are required
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