Abstract
The present study was designed to investigate the effects of microRNA-21 (miR-21) on orthodontic tooth movement. The orthodontic tooth movement model was established in C57BL/6 and miR-21-/- mice with or without implantation of activated T cells. Histological and histomorphometrical analyses were performed by hematoxylin-eosin staining. Tartrate-resistant acid phosphate staining was used to analyze the osteoclast numbers during tooth movement. Enzyme-linked immunosorbent assay, reverse transcription polymerase chain reaction, and immunohistochemistry analysis were used to examine the expression of RANKL and OPG. In miR-21-/- mice, the distance of tooth movement was retarded, the osteoclast number was decreased, and serum RANKL expression was strongly reduced. MiR-21 promoted the secretion of RANKL from activated T cells. Furthermore, activated T cells could partially rescue the decreased orthodontic tooth movement distance in miR-21-/- mice. MiR-21 was shown to promote orthodontic tooth movement by modulating the RANKL/OPG balance in T cells. The impact of miR-21 on tooth movement was better elucidated, furthering our understanding of its role and clinical applications in orthodontics.
Published Version
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