Micropropagation studies of sweet orange (citrus sinensisOsbeck) cv. Blood Red

Abstract

This study was conducted to study in vitro technique, micropropagation of sweet orange (Citrus sinensis Os beck) cv. Blood Red. The impact of plant growth regulators on axillary bud proliferation in Blood Red was studied by using MS basal medium added with BAP (1.0, 3.0, 5.0 and 7.0 mg l−1) and NAA (0.1, 0.5, 1.0 and 1.5 mg l−1) for rooting IBA and NAA (0.1, 0.5, 1.0 and 2.0 mg l−1 each) either alone or in combinations. The maximum percent success, number of shoots, length of shoot and minimum number of days taken to bud break were obtained with BAP (3.0 mg l−1) and NAA (0.5 mg l−1). For rooting, lower concentrations of NAA and IBA up to 0.5 mg l−1 were found better in case of maximum percent success, number of roots, length of roots and minimum number of days taken to root initiation as compared to higher levels.