Micropropagation of Stevia rebaudiana plants

Marta Teresa Rokosa,Danuta Kulpa

doi:10.1590/0103-8478cr20181029

Marta Teresa Rokosa, Danuta Kulpa

Open Access

https://doi.org/10.1590/0103-8478cr20181029

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Journal: Ciência Rural	Publication Date: Jan 1, 2020
Citations: 6	License type: CC BY 4.0

Affiliation: West Pomeranian University of Technology

Abstract

ABSTRACT: The aim of the study was to develop optimum composition of plant growth regulators in media for the propagation and rooting of shoots of stevia (Stevia rebaudiana Bertoni) in in vitro cultures. Single-node shoot fragments obtained from plants propagated on MS medium were placed onto media supplemented with: BAP, 2iP and KIN at concentrations: 0.5, 1, 2 and 5 mg∙dm-3, whereas at the rooting stage with addition of: IAA, IBA and NAA at concentrations 1, 2, 4 and 8 mg∙dm-3. The highest number of shoots and leaves was reported for plants propagated on MS medium enriched with 0.5 mg∙dm-3 BAP. The greatest number of the longest roots was developed by stevia on the MS medium enriched with 1 mg∙dm-3 IAA.

Highlights

Stevia rebaudiana Bertoni is a plant belongs to the Asteraceae family
Propagation stage The study allowed to demonstrate the addition of BAP and 2iP cytokinins influenced the increase in the number of side shoots
Plants propagated on media with the addition of cytokinins produced a higher number of leaves relative to control plants (9.60)

Summary

Introduction

Stevia rebaudiana Bertoni is a plant belongs to the Asteraceae family. It is a shrub growing to 80 cm height, having leaves with a lanceolate shape. The stem is woody, the flowers are abundant and possess white-violet color (LEMUS-MONDACA et al, 2012). Its leaves contain diterpene glycosides, such as steviosides, rebaudiosides, rubosides steviolbiosides and dulcosides, the sweetness of which is up to 300-fold higher than traditional sugar and it is known as sweet leaf (BONDAREV et al, 2001, DASI et al, 2011, GANTAIT et al, 2015, GEUNS, 2003, LEMUS-MONDACA et al, 2012, SIVARAM & MUKUNDAN, 2003). Stevia is used as a healthier substitute for cane or beet sugar, commonly used to sweeten many dishes. The plant has been used in the area of its origin

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