Abstract
Rhynchostylis gigantea is a wild orchid species commonly grown. Flowering at the traditional Tet holiday, the flower season always blooms in December of the lunar calendar. The flower has a faint aroma, so it is called Nghinh spring and is a kind of orchid with a national soul and national essence. Rhynchostylis gigantea is found in many areas in Vietnam as it is distributed in the South Central Highlands, especially the areas bordering Laos and Cambodia at low altitudes <600m. Rhynchostylis gigantea is a type of heat-resistant orchid, with suitable temperatures for orchid being from 26-30oC. Rhynchostylis gigantea species have many different colors such as red, white, shrimp brick color, blue and pink. The single-member tree does not assume pseudobulbs, grows vertically, has many pneumatophores and grows straight from the stem. Rhynchostylis gigantea grows slowly and regenerates with seeds. Due to high genetic diversity, it is difficult to maintain the parent’s characteristics. Applying techniques of somatic embryo culture in conservation and development of the Rhynchostylis gigantea tree to meet the increasing demand for seedlings is urgent.
Highlights
Orchidaceae make up about 10% of angiosperm species
On the MS medium supplemented with NAA (0.1 mg/l) and TDZ (0.1 mg/l) protocorm-like body (PLB) production of 78% was stimulated
Young leaves were cultured as PLB formation on the MS medium, TDZ (0.1 mg/l), having a PLB production rate of 100% and reaching of 8.6 PLB/sample
Summary
Orchidaceae make up about 10% of angiosperm species. An orchid is a large species, with diversified shape, size, and color, adapted to a large ecosystem, and is important in the flower industry with 20,000-30,000 different species (Griesbach, 2003).Cell culture has been used to rapidly multiply orchid species that are suitable for different culture conditions. Orchidaceae make up about 10% of angiosperm species. An orchid is a large species, with diversified shape, size, and color, adapted to a large ecosystem, and is important in the flower industry with 20,000-30,000 different species (Griesbach, 2003). Cell culture has been used to rapidly multiply orchid species that are suitable for different culture conditions. The protocorm-like body (PLB) is the first embryogenic organism. Rhynchostylis gigantea orchid is a single shoot species that are commonly found in Southeast Asia (Vietnam, Laos, Thailand). The propagation of Rhynchostylis gigantea orchid using protocorm can be done through culturing shoots, Rhynchostylis gigantean young leaf, Rhynchostylis retusa root, or PLB regeneration directly from thin cell layer culture (Le et al, 1999)
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