Abstract
Chukrasia tabularis belongs to the Meliaceae family, which is native to many Asian countries, including Vietnam. It is a precious wood species with beautiful wood grain, light-loving tree with evergreen leaves, widely grown in many places Cameroon, Costa Rica, Nigeria, Puerto Rico, South Africa, United States, China ... for many different purposes: planting economic forests, alternative forests, protective forest belts and urban greenery. In Vietnam, Chukrasia tabularis are usually distributed naturally in the northern, central highland and southern provinces. From Nghe Tinh onwards, there are many varieties such as Dong Nai Chukrasia tabularis (Chukrasia tabularis var. Dongnaiensis), Con Dao Chukrasia tabularis (C. tabularis sp.), small fruit Chukrasia tabularis (C. tabularis var. Microcarpa), and hairy Chukrasia tabularis (C. tabularis var. Velutina). Chukrasia tabularis often grow on well-drained soil, at an altitude of 300-800 m.asl, the annual average temperature is from 2-43oC, rainfall is 1,800-3,800 mm. Singing and development occurs in high limestone mountains, meaty soil, mixed meat, nutrient poor sandy soil at an altitude of less than 100 m. The tree is regenerated by seed but grows slowly and with high genetic diversity. Techniques of somatic embryo culture are applied to preserve and develop Chukrasia tabularis plants with urgent needs.
Highlights
Traditional micropropagation of woody plants has a problem where the tissue culture plants often grow slowly and the costs of labor, to produce the seedlings and the seedlings cost themselves, are high (Murashige et al, 1962)
Cytokinin is necessary for lateral shoot development, for some plants that need to be combined with auxin, and in culture medium supplemented with coconut water stimulates increased effectiveness of cytokinin and auxin effects
Embryonic callus arises on the cut of leaf and stem samples and soon reaches the maximum weight on the 20th day
Summary
Traditional micropropagation of woody plants has a problem where the tissue culture plants often grow slowly and the costs of labor, to produce the seedlings and the seedlings cost themselves, are high (Murashige et al, 1962). Asexual embryo-propagation system (Evans et al, 1981) addresses the above-mentioned problems with certain advantages such as the rapid multiplication in the form of asexual embryos which is a form of differentiation with a high regeneration coefficient, low cost of labor dynamic keeping the price down (Kurata et al, 1992). The starting material in asexual embryo culture plays a role in securing parental traits and maintaining a high regeneration coefficient for a long time (Lu et al, 2004). A suitable clonal culture medium plays an important role in embryonic growth and differentiation (Evans et al, 1981). Asexual embryo culture, and asexual embryo regeneration is the first barrier in somatic embryo technology (Jain et al, 1995; Sondahl et al, 1977). We study the fast multiplication of chukrasia tabularis A.Fuss by somatic embryo technology
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