Abstract
The hybrid Rutgers red leaf peach was obtained as a part of the selection programme of the Fruit Growing Institute, Plovdiv. Its leaves are dark red to violet in colour and it exhibits moderate growth. The present study was established to develop a micropropagation protocol for this hybrid. The influence of modified Murashige and Skoog (MS) and Quoirin and Lepoivre (QL) media on the multiplication rate and growth of the generated shoots was studied. The nutrient media were enriched with 2.5 µM 6-benzylaminopurine (BAP), 0.005 µM indole-3-butyric acid (IBA), 0.3 µM gibberellic acid (GA3) and varying concentrations (separately and in combination) of sucrose and sorbitol. The nutrient media for root induction contained all the components according to MS, with 25% macroelements and varying concentrations of the auxin IBA (0 µM, 1.5 µM, 2.5 µM and 5.0 µM). The best multiplication rate was achieved on media supplemented with sucrose (15 g L-1) and sorbitol (15 g L-1). The highest rooting percentage was obtained on a medium containing 1.5 µM IBA. After acclimatization a number of plants were selected and used for field tests as peach rootstocks and/or ornamental plants.
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