Abstract
<p>Shoot-tip multiplication of the medicinal species - <em>Plantago asiatica</em> was carried on MS medium with IAA and BAP or kinetin. Best results in micropropagation were achieved by adding 0.1 mg/dm<sup>3</sup> IAA and 1 mg/dm<sup>3</sup> BAP. After 6 weeks shoots were transferred to MS medium for rooting. The resulting plantlets were transferred after 8 weeks into pots and after a period of adaptation into the ground (field culture).</p><p>The species <em>Plantago asiatica </em>was propagated in vitro by shoot-tip multiplication for the first time.</p>
Highlights
Among over 200 described species of the Plantaginaceae family only several have been cultured in vitro so far: P. asiatica L. (Tu Yisheng 1996; Andrzejewska-Golec 1998a, b; Makowczyñska and Andrzejewska-Golec 2000), P. lanceolata L. (Skrzypczak et al 1995; Budzianowska and Skrzypczak 1998; Fons et al 1998, 1999; Budzianowska 2001), P. major L. (Brimer 1988; Martin-Cordero et al 1996; Mederos et al 1997/1998), P. maritima L. (Chang and Locy 1996), P. media (Kunvari unpublished data), P. ovata Forsk. (Wakhlu and Barna 1988, 1989; Pramanik et al 1994-1996; Chowdhury et al 1996; Barna and Wakhlu 1998).P. asiatica species related to P. major grows only in the Far East countries
The seeds of P. asiatica were obtained from the Botanical Gardens, Faculty of Science, University of Tokyo
They were germinated in aseptic conditions on Murashige and Skoog medium (MS) supplemented with 2.0 mg/dm3 kinetin, 1 mg/dm3 gibberellic acid (GA3) and 0.7% Difco Bacto-Agar
Summary
Among over 200 described species of the Plantaginaceae family only several have been cultured in vitro so far: P. asiatica L. (Tu Yisheng 1996; Andrzejewska-Golec 1998a, b; Makowczyñska and Andrzejewska-Golec 2000), P. lanceolata L. (Skrzypczak et al 1995; Budzianowska and Skrzypczak 1998; Fons et al 1998, 1999; Budzianowska 2001), P. major L. (Brimer 1988; Martin-Cordero et al 1996; Mederos et al 1997/1998), P. maritima L. (Chang and Locy 1996), P. media (Kunvari unpublished data), P. ovata Forsk. (Wakhlu and Barna 1988, 1989; Pramanik et al 1994-1996; Chowdhury et al 1996; Barna and Wakhlu 1998).P. asiatica species related to P. major grows only in the Far East countries Among over 200 described species of the Plantaginaceae family only several have been cultured in vitro so far: P. asiatica L. (Tu Yisheng 1996; Andrzejewska-Golec 1998a, b; Makowczyñska and Andrzejewska-Golec 2000), P. lanceolata L. (Skrzypczak et al 1995; Budzianowska and Skrzypczak 1998; Fons et al 1998, 1999; Budzianowska 2001), P. major L. P. asiatica species related to P. major grows only in the Far East countries P. asiatica (Plantaginis herba and Plantaginis semen) are included in the Japanese Pharmacopoeia XIV (2001). This species has been used in the food industry (Kanamaru 1994; Tokumaru and Tokumaru 1999) and cosmetics production (Ito 1996)
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