Abstract
Plant regeneration of Anubias barteri var. Nana was achieved through organogenesis in shoot tip cultures. Multiple shoots were induced from cultured shoot tips on a modified MS (Murashige and Skoog, 1962) medium supplemented with BA and kinetin. The maximum green shoot numbers were best obtained on MS medium containing 3 mg/L BA with 5 shoots. Rooting in all regenerated shoots was promoted on MS medium devoid of plant growth regulators or kinetin singly. Acclimatization and survival when transferred to field conditions were shown to be 100% in the regenerated plants. Cytological and flow cytometric analyses of the mother plants and in vitro grown plants derived from 5 years old cultures showed no differences in ploidy level, they were all diploid (2n = 2x = 48) with a 2C peak indicating that ploidy alteration did not occur.
Highlights
The genus Anubias of the family Araceae is divided into many varieties such as Anubias barteri var
This study investigated an efficient protocol for A. barteri var
The results of in vitro organogenesis in A. barteri var Nana are shown in Tab. 1
Summary
The genus Anubias of the family Araceae is divided into many varieties such as Anubias barteri var. The chromosome number of A. barteri var. Flow cytometry is being used to analyze DNA content in a number of plant species; which require only a small amount of tissue and is non-destructive. The study investigated the effects of plant growth regulators on number of chromosomes using flow cytometry as rapid methods for detecting ploidy levels in regenerated plants.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
