MICROPROPAGATION OF ORTHOSIPHON ARISTATUS THROUGH INDIRECT AND DIRECT ORGANOGENESIS

Abstract

Orthosiphon aristatus is a herbal and ornamental herb from Lamiaceae family that has long been used as a diuretic for alleviating uric acid disease, gallbladder and kidney stone. Conventional propagation of this plant by stem cutting method faces major challenges notably due to the defect in roots formation, while the demand for this plant is high. This research aimed to establish a protocol for optimal micropropagation of O. aristatus through indirect and direct organogenesis method. Calli induction on MS agar medium supplemented with 2,4-D 0.5 mg L-1 and kinetin 0.3 mg L-1 resulted in 4.32 ± 1.49 g fresh weight of calli. Regeneration of shoots was successfully achieved on MS with 2 mg L-1 BAP with 47 shoots from a single clump of calli. MS0 medium was found to be the best in inducing roots formation. Meanwhile, multiplication through direct organogenesis was optimally performed on MS supplemented with 2 mg L-1 BAP with 9.00 ± 4.42 new shoots produced in 8 weeks after cultured. In conclusion, optimal multiplication shoots of O. aristatus has been achieved through callus formation and direct organogenesis.