Abstract
An experiment was conducted to steady the effect of L-Tryptophan and salicylic acid on callus induced on leaf explants of Catharanthus roseus. Callus induction was achieved by culturing true leaves of the plant on Murashige and Skoog (MS) medium supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin. The best medium to maintain callus was MS medium supplemented with 0.5 mg /L 2,4-D and 1.5 mg / L Kin. Different levels added to MS medium for each L-Tryptophan 0,200,300or400 mg /L and salicylic acid 0, 0.5,1or1.5mg /L. The medium supplemented with 30 g/ L sucrose was used as a control. Results showed the medium supplemented with 200 mgL of L-Tryptophan gave the highest quantity of Vincristine reached 48.66 µg/100 fresh weight of callus. MS medium content at the concentration 1 mg L of salicylic acid gave the highest level of Vinblastine recording 50.98 µg/100 fresh weight of callus. While MS medium supplemented with 0.5 mg /L of salicylic acid gave the highest level of Serpentine 24.76 µg/100 fresh weight of callus. The concentrations of active compound derived from plant leaves were much less than the concentrations produced by the callus grown in vitro. The concentration of Serpentine was 0.059 while Vinblastine was 0.183 and the concentration of Vincristine was 0.064.
Highlights
Influence of L-Tryptophan and salicylic acid on secondary metabolites production from leaves induced callus of Catharanthus roseus L.G.Don in vitro
Salicylic acid ،L-Tryptophan ، اَذٔل انكهٌٕذ،ٌٔ َباث عٍٍ انبض:انكهًاث انًفخاحٍت Abstract An experiment was conducted to steady the effect of L-Tryptophan and salicylic acid on callus induced on leaf explants of Catharanthus roseus
Callus induction was achieved by culturing true leaves of the plant on Murashige and Skoog (MS) medium supplemented with 0.5 mg /L 2,4-D and 1mg / L Kin
Summary
Influence of L-Tryptophan and salicylic acid on secondary metabolites production from leaves induced callus of Catharanthus roseus L.G.Don in vitro. انُخائش ٔانًُالشت حأرٍش L-Tryptophanفً إَخاس انًشكباث انزإٌَت يٍ انكانس ٠لاؽع ِٓ ٔزبئظ عذٚي ( )1إْ ٕ٘بٌه فشٚلب ِؼٕ٠ٛخ ف ٟرشو١ض اٌّشوجبد اٌضبٔ٠ٛخ اٌّذسٚعخ ػٕذ إػبفخ رشاو١ض ِخزٍفخ ِٓ اٌجبدا L- Tryptophanإٌ ٝاٌٛعؾ اٌغزائ .ٟاْ اػٍ ٟوّ١خ ٌّشوت اٌـ Serpentineلذ ؽظٍذ ػٕذ إػبفخ اٌزشو١ض ٍِ200غٌُ/زش ِٓ اٌجبدا ٚثٍغذ ِ 22.26ب٠ىشٚغشاَ ٍِ100 /غُ ٚصْ ؽش ٞوبٌظ ثّٕ١ب أػطذ ِؼبٍِخ اٌّمبسٔخ الً لّ١خ ِٓ اٌـ Serpentineثٍغذ 5.98
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