Abstract
Orchids are one of the most popular plants in the world and among them the species of Phalaenopsis have the most sales on the global market. Because of its difficult propagation, micropropagation has been suggested recently. In the current study, the leaves obtained from in vitro culture of flower stalk nodes were used as explants and were cultured on MS medium with different concentrations of NAA, BA and TDZ. Protocorm-like bodies (PLBs) were produced and transferred to medium without growth regulators. Finally, the adaptation of plants was evaluated in a medium of cocopeat + coal (3:1 v/v) and another medium of cocopeat + charcoal + LECA (2:1:2 v/v). Results showed that the highest percentage of active samples was 100% which could regenerate the PLBs by being treated with 4 mg/l TDZ. The lowest active samples (60%) were those treated in the medium with 4 mg/l BAP + 0.5 mg/l NAA. The highest PLBs per explant (50.65) were obtained in the medium supplemented with 15 mg/l BAP + 3 mg/l NAA. Best acclimation (90%) of plants was obtained when medium of cocopeat + charcoal + LECA (2:1:2 v/v) was used. According to the results of the current experiment, the MS culture medium containing 15 mg/l BAP + 3 mg/l NAA was thereby considered as the best medium for Phalaenopsis micropropagation.
Highlights
Orchids are comprised of 800 genera and approximatively 25,000 species
Chen and Chang (2004) examined the effects of TDZ and NAA on the embryogenesis of protocorms resulted from culturing the seeds of P. amabilis ‘Formosa’
The extra moisture on the explants was dried and the explants were exposed to the flow of air under the hood used for tissue culture
Summary
Orchids are comprised of 800 genera and approximatively 25,000 species. They are one of the oldest and most highly developed families of plants. Tokuhara and Mii (1993) observed that the best combination of plant growth regulators which can be used for in vitro media culture was 20 mg l-1 BAP and 0.1 mg l-1 NAA, so as to yield the best result of producing the most protocorms growing from the buds of flower stalks. Chen and Chang (2004) examined the effects of TDZ and NAA on the embryogenesis of protocorms resulted from culturing the seeds of P. amabilis ‘Formosa’ They found that the best condition for embryogenesis occurred in half-strength MS medium with 3 mg l-1 TDZ. The effects of BAP, NAA and TDZ were examined on the direct embryogenesis and proliferation of clonal plants of the studied orchid species. The explants used in the research were leaves that were obtained by tissue culturing the nodes located on flower stalks
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
