Abstract
Grapes and strawberries are major fruit crops with a dynamic, fast-growing global market. In addition to conventional techniques, biotechnological tools allow the preservation of valuable genotypes of both crops and the large-scale propagation of high-quality material. We have developed new protocols for expeditious and reliable micropropagation of grapes and strawberries cultivated in south Russia. In vitro cultivation on semisolid media was combined with rapid propagation in bioreactors. A six-week cycle of propagation in a bioreactor resulted in a 300-fold increase in fresh mass, with a propagation rate of ~5 in grapes and ~20 in strawberries. Genetic analysis using the inter simple sequence repeat (ISSR) DNA fingerprinting technique confirmed the full uniformity of the regenerants. The results of this research will support the local horticultural industry with fast and efficient propagation systems of new breeding lines, introduced varieties, and elite cultivars.
Highlights
Agronomy 2022, 12, 308. https://Grapes and strawberries are major fruit crops with a dynamic, fast-growing global market
We have developed protocols for expeditious and reliable micropropagation of grapes and strawberries cultivated in south Russia
100% of the grape plantlets formed roots after 2–4 weeks of supplemented with indolylbutyric acid (IBA)
Summary
Agronomy 2022, 12, 308. https://Grapes and strawberries are major fruit crops with a dynamic, fast-growing global market. The whole fruit, skin, leaves, and seeds of the grape plant are used in cosmetics and the pharmaceutical industry [2]. Crop improvement and timely monitoring of product quality and propagation of the highest quality plant material are critical for grape and strawberry production. Vegetative propagation in strawberries from plugs or bare-rooted units ensures the retention of additive and non-additive genetic traits [6]. In addition to conventional techniques, plant biotechnology has been used to preserve valuable genotypes of both crops, propagate high-quality material on a large scale, perform physiological studies, and obtain genetically transformed types. The application of tissue culture of strawberries and grapes provides technical support for the industrial production of these important crops [7,8,9,10]
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