Abstract
The aim of this investigation was to study micropropagation of Almond GF rootstock on in vitro. Explants were cultured in MS medium containing 5 concentrations of 6- Benzylaminoporine (BAP) 0.1, 0.6, 1, 1.5 and 2 mg 1-1 along with control treatment. Each treatment includes 5 replications. After 5 weeks of culture, the results showed that the number and length of shoots in treatment containing 1 mg 1-1 BAP was more than the other treatments and control. The number of shoot that was obtained in treatment of 1 mg 1-1 BAP was 2.98 (Table 1). The lowest number of shoot was 0.4 due to control treatment. For rooting, explants with 3-5 cm length were transferred to different rooting treatments (Table 2). The results revealed that the best rooting was obtained with treatment 3 mg 1-1 IBA. Number and length of roots were 2.167 and 11.53, respectively. Also results showed that putting explants in dark for 1 week is more effective than light.
Highlights
GF is a hybrid of Prunus amygdalus × P. persica and is the most commonly used rootstock for peach orchards (Antonopoulou et al, 2007)
Shoot branching depends on the initiation and activity of axillary meristems, which are hormonally controlled by cytokinin (Dobranszki and silva, 2010)
The ability of plant tissue to form adventitious roots depends on interaction of many exogenous and endogenous factors, including hormone
Summary
GF is a hybrid of Prunus amygdalus × P. persica and is the most commonly used rootstock for peach orchards (Antonopoulou et al, 2007) This rootstock is tolerant to Fe deficiency and specially suited to soils with poor fertility, low water availability and high CaCo3 content (Monticelli et al, 2000). Exogenous auxins are only required at an early stage to stimulate emergence of new formed roots (Dobranszki and silva, 2010). It has been reported the best hormone for rooting was IBA (0.5 mg 1-1) (Vaez-Livari et al, 2005). The positive effects of Darkness have been reported on root number, root length (Vaez-Livari et al, 2005)
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