Abstract
The aim of this study is to optimize in vitro multiplication of Coffea arabica cvs 'Benan',' Burai',' Odayni'and 'Odayni- Bayat'. Seeds germinated on modified quarter MS medium with 1.0 g l−1 activated charcoal gave thehighest germination percentage of the four cultivars under study. BA at 8.0 mgl-1 was the most effective concentrationwith an average of 14 shoots per explant, resulting in better and morphologically superior microshoots in 'Benan' cv.However 'Burai' ,'Odayni' and 'Odayni- Bayat' cultivars, showed the highest number of shoots when they were grownon MS medium supplemented with BA at 4.0, 2.0 and 6.0 mgl-1 respectively. Root formation was 100% in response tothe application of IAA, IBA and /or NAA to the culture media for the four cultivars. Half MS supplemented with NAAat 2.0, 1.0 and 3.0 mgl-1 was found to be a suitable medium for root induction in excised micro-shoots, of 'Odayni','Burai' and 'Odayni-Bayat' cultivars, respectively. The plantlets were successfully acclimatized in the greenhouse;consequently the survived plants reached 100%.
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