Abstract
A plant micropropagation system has been developed with the capacity of allowing periodic, automatic, aseptic monitoring of culture medium composition. The system was able to sample automatically culture medium from a liquid/membrane bioreactor without compromising asepsis or disturbing plant tissue. Media samples were analyzed to determine sucrose and dextrose concentrations. Bioreactor media was automatically replenished to compensate for volumes lost due to sampling. For a 28-day culture period using Stage 1 Nicotiana tabacum (tobacco) explants as a model culture system, media sucrose concentration decreased with a corresponding increase in dextrose concentration and tissue fresh weight.
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