Abstract
A whole plant liquid/membrane micropropagation bioreactor system was modified to allow the culture process to operate semicontinuously. The medium composition control (MCC) system allowed bioreactor medium to be sampled automatically at 3-h intervals and an aliquot analyzed for sucrose and dextrose concentration. Data obtained were used as the input to a three-state on/off control system which maintained medium sucrose within a preset threshold of 5% of the desired concentration and medium volume levels within a 2.5% threshold. The MCC system performance testing, without explant tissue, showed it to respond within these design limits when disturbances and setpoint changes were imposed on the system. Stage 1 Nicotiana tabacum (tobacco) explants cultured in the MCC system were found to have both large fresh weight gains and prolific shoot production. The MCC-grown explants showed increased tissue fresh weight gains over previous bioreactor studies including treatments on conventional agar-based medium, on static-liquid medium, in a liquid/membrane bioreactor with seven-day fresh medium replacement and in a liquid/membrane bioreactor equipped for monitoring sucrose concentrations.
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