Microplastics affect the ecological stoichiometry of plant, soil and microbes in a greenhouse vegetable system

Abstract

Microplastic (MP) pollution is a growing global issue due to its potential threat to ecosystem and human health. Low-density polyethylene (LDPE) MP is the most common type of plastics polluting agricultural soils, negatively affecting soil-microbial-plant systems. However, the effects of LDPE MPs on the carbon (C): nitrogen (N): phosphorus (P) of soil-microbial-plant systems have not been well elucidated. Thus, we conducted a pot experiment with varying LDPE MP concentrations (w/w) (control without MPs; 0.2 % MPs (PE1); 5 % MPs (PE2); and 10 % MPs (PE3)) to study their effects on soil-microbial-plant C-N-P stoichiometry. Soil C:N ratio increased 2.3 and 3.4 times in PE2 and PE3, respectively. Soil C:P ratio increased 2.2 and 3.6 times in PE2 and PE3, respectively. Soil microbial C:N ratios decreased by 46.2 % in PE1, while C:P ratios decreased by 59.2, 38.6, and 67.9 % in PE1, PE2, and PE3, respectively. Soil microbial N:P ratio decreased in PE1 (17.2) and PE3 (59.1 %). MPs increased shoot C content and C:N ratios, particularly at the 5 % MP addition rate. MP addition altered dissolved organic C, N, and P concentrations, depending on the MP addition rate. Microbial community responses to MP exposure were complex, leading to variable effects on different microbial groups at different MP addition rates. Structural equation modeling showed that MP addition had a direct positive effect (β = 0.96) on soil C-N-P stoichiometry and a direct negative effect (β = −1.34) on microbial C-N-P stoichiometry. These findings demonstrate the complex interactions between MPs, soil microorganisms, and nutrient dynamics, highlighting the need for further research to better understand the ecological implications of MP pollution in terrestrial ecosystems.