Microplastic contamination of packaged spirulina products.

Abstract

Microplastic (MP) contamination in commercially soldspirulina products has not been previously investigated. In this study, 29 spirulina samples in various packaging types were purchased from different brands and origins to assess the presence of MPs. Microplastic analysis was conducted using microscopic and μ-Raman techniques. To ascertain whether the content is indeed spirulina and make a comparison with the MP level, C-Phycocyanin levels were also analyzed. A total of 251 MP-like particles were observed. Out of the 29 examined packaged spirulina brands, 26 showed potential MPs upon visual inspection, with 35 particles confirmed as MPs (73% of the analyzed particles). The mean abundance of MPs was estimated at 13.77 ± 2.45 MPs/100g dw. Powdered spirulina had a higher but not statistically significant MP abundance (17.34 ± 4.22 MPs/100g dw) compared to capsule/tablet forms (10.43 ± 2.45 MPs/100g dw). Fragments accounted for 38.3% while fibers constituted 61.7% of the identified MPs, with sizes ranging from 0.07 to 2.15mm for fragments and 0.19 to 5.691mm for fibers. The color distribution of MPs in spirulina samples was predominantly blue (52.8%), followed by black (25.4%), white (10.9%), and others (10.9%). Ten synthetic polymers and cellulose were identified through μ-Raman analysis, with polypropylene (31.6%) and polystyrene (8.3%) being the most prevalent. The correlation between C-Phycocyanin and MPs concentrations, was not found statistically significant. The abundance and composition of MPs were found to be influenced by packaging and processing stages. Identifying potential sources of MPs in spirulina products and evaluating their risks to human health is crucial.