Abstract
FSM16 mesoporous silicate and its chemically modified samples were synthesized. Then the relevant nanobiocatalysts consisting of Fe(III)protoporphyrin(IX) (Hemin, Fe(III)PPIX), microperoxidase-11 and horseradish peroxidase were obtained via direct immobilization of the biocatalysts in the nanopores of amine modified FSMs. The prepared catalysts were characterized by XRD, ASAP and diffuse reflectance UV/Vis techniques. The performances of the obtained peroxidase model nanostructures were evaluated by some typical test reactions, such as oxidation of ABTS, ortho-methoxyphenol (guaiacol) and peroxidatic synthesis of indophenol and N-antipyryl- p-benzoquinoneimine. Kinetic parameters including initial reaction rates, rate constants, V max, turnover number, Michaelis constant and catalytic efficiency were obtained and compared to those of Fe(III)PPIX/MCM41 (as a blank) and homogeneous native horseradish peroxidase (HRP). Results showed that MP-11-NH 2-FSM16 nanobiocatalyst is able to mimic horseradish peroxidase with a K m value of 55.45 ± 1.29 μM with respect to ABTS and guaiacol (as the reducing substrates of HRP). The prepared nanobiocatalysts with high catalytic efficiencies about 10 8 M −1 min −1 showed high peroxidatic activity for oxidation and conversion of aromatic substrates.
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